FastQCFastQC Report
Fri 10 Jun 2016
G95-M2_TGACCA_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M2_TGACCA_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6047762
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCG2257.952281E-414.4661227
CCGGAAT12700.012.4512321
CCGGGAT14650.012.381241
CGGTCGA2750.003597153611.8376794
CCGAAAT8750.011.6936951
CGGGAAT8551.8189894E-1211.4232661
CTCGAAT10400.011.1800511
CGGGTAT5557.3429874E-710.8940031
CCCGGTT10003.6379788E-1210.2319831
CGCCAAT8703.1286618E-1010.1571421
CACGATT6901.0704571E-710.1106561
CCGGGTT15650.010.1041961
CGCGCAT4156.435431E-410.0862921
CGCAAAT4207.184159E-49.9662181
GTCGCAT4701.8294521E-49.89553451
CCGCATT8101.0712029E-89.7611511
CTCGATT7654.6144123E-89.7273751
CGCAGAT14900.09.6763731
AATCGTA4359.918954E-49.6217595
CGGCAAT7958.352072E-89.3603051
Produced by FastQC (version 0.11.2)