FastQCFastQC Report
Fri 10 Jun 2016
G95-M2_TGACCA_L002_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M2_TGACCA_L002_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6047762
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAT2457.9198764E-414.4738821
CGCAATT2600.001241115213.638851
CTCGTAT4051.684489E-512.5082931
CGATCAC8101.364242E-1011.5742943
CTCGGTT11900.011.4939651
CGCGTGT5957.1752875E-610.2168581
CGCCGTT4000.002652900810.1317181
CGCCTGT18250.09.9929271
TCGGATC8851.126682E-89.7310282
CGGGTAT5856.64503E-59.5255461
CCGGGTT14400.09.4984851
CCGGACT10151.0895747E-99.4828881
CGCCCAT13950.09.4417441
GATTCGT5603.3470412E-49.318355
AATCGTA5050.00135371789.2998975
ACGATCA8254.153626E-79.2106522
ATCTAAC17450.08.9713326
GAATCGT5250.00192839998.9456164
CCGGGAT15400.08.88170051
CTCGGAT13957.2759576E-128.7154561
Produced by FastQC (version 0.11.2)