FastQCFastQC Report
Fri 10 Jun 2016
G95-M3_ACAGTG_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M3_ACAGTG_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8852061
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT5953.6379788E-1214.2905061
CGCCAAC14950.010.7431041
CCGGAAT20750.010.6997061
CGCGATG5901.3252757E-610.40551952
CCGGCAT16800.09.8412641
CCGCGAT6303.0583415E-69.7475361
CGACGGT5355.3719836E-59.7124991
CTCGGAT22400.09.7006731
CCGAAAC14150.09.6812991
CTCGATT10751.0913936E-119.6673241
CGCCATT19550.09.6650761
GCGGTAT6454.1282074E-69.520851
CCGCGGT9055.0731614E-99.3954151
CGATCAC13950.09.3307533
CCGGGAT20550.09.19475651
CTCGTTT15950.09.1810651
CGCAAAC10002.9413059E-98.9752321
CCGGAGT20550.08.9648881
CGCTTGT13201.8189894E-128.9466031
TGCGATC11152.4738256E-108.8944042
Produced by FastQC (version 0.11.2)