FastQCFastQC Report
Fri 10 Jun 2016
G95-M3_ACAGTG_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M3_ACAGTG_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8485754
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAT8850.012.0867671
CCGGAAT18500.011.0613091
ACGATCA10900.010.6665442
CCGGTAT9601.8189894E-1210.6580321
CCGAAAC14000.010.6303491
CGCGCAT4601.4731423E-410.1103871
CCCGGTT16400.09.925441
CGCCAAT11350.09.8342441
CCGGGTT17500.09.8330731
CCGGGAT20600.09.7079341
CCCGGAT21200.09.6525561
CCGACAT16550.09.5544681
CACGGAT18250.09.4289731
CGCGTGT8401.9412255E-89.4122881
CCCGTAT8009.204268E-89.3015561
CTCGAAT14000.09.3015561
CTCGGAT22950.09.1191721
CGGAAAT13300.09.0917461
CGATCGT5205.048511E-48.9433814
CGCAAAT7352.7393544E-68.85862351
Produced by FastQC (version 0.11.2)