FastQCFastQC Report
Fri 10 Jun 2016
G95-M3_ACAGTG_L002_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M3_ACAGTG_L002_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8485754
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT2050.00449562114.6001221
ACGATCA9950.011.0295732
CGACGGT5151.9384399E-510.6547811
CGGCGAT6653.041525E-69.751711
CGACGAT4800.00128503529.3532041
CGCAGAT19000.09.1891131
CGATCAC12951.0913936E-119.11267953
CGCCGTT7157.647146E-69.0697731
TCAATCG7408.272809E-69.0136283
CCGAAAT12205.2750693E-118.9954311
CGCTAAT4450.0072606448.9678661
CGCCCAT19050.08.9031371
TCGGATT10351.985245E-88.6754352
CACGATC15550.08.6614881
CGCCAAT10402.1549567E-88.6337261
CTCGAAT13202.6557245E-108.3139581
CCGGAAC22050.08.1442871
TCGAATC10452.134002E-78.115062
CGAGAAT19800.08.062021
CCGTAAT9302.8996255E-68.0457671
Produced by FastQC (version 0.11.2)