FastQCFastQC Report
Fri 10 Jun 2016
G95-M4_GCCAAT_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M4_GCCAAT_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7166762
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAT3606.4917367E-714.4362411
ACGATCA10250.012.4409352
CGCCGTT5403.1897798E-812.2489331
CGATCAC10600.011.8411973
CCGGCAT13850.011.5982671
CGCATAT5555.9956983E-711.0666041
CCGGAAT15150.010.6030371
CCGGGAT16600.010.5307091
AATCGAC4451.0582701E-410.4464465
CACGATC15200.010.257331
CGCAATC4701.5667622E-410.0488242
CCGGAAC18650.09.6265081
CCCGGAT15750.09.5991631
CCGGCTT18300.09.5524461
GTCGGTA6504.5445486E-69.4491761
CGCTAGT4550.00129491069.3453391
CCGCAAT6605.5181445E-69.3060071
CTCGGTT14000.09.1117071
ATCGAGT8201.3543286E-79.0705746
CGGAAAT10751.1550583E-98.7899311
Produced by FastQC (version 0.11.2)