FastQCFastQC Report
Fri 10 Jun 2016
G95-M4_GCCAAT_L001_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M4_GCCAAT_L001_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7166762
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAT5302.8921932E-1015.0553811
ACGATCA10100.012.113472
CGTCAAT9055.4569682E-1111.1681531
CGATCAC11000.011.0846033
CCGCAAT7454.6009518E-810.710541
CACGATC14400.010.3436041
CGCACAT10003.4197E-1010.1071791
CTCGAAT11102.1827873E-1110.0640481
CGCGGAT5351.7430817E-49.9431181
CGATCTA4607.3602126E-49.9399984
CCGGAAT15650.09.8573471
CGACAAT6551.2578454E-59.7457731
TCTACGC6501.9567888E-59.3888317
CGGAAAT11908.54925E-119.3874731
CTCGTAT5703.2964337E-49.3325761
GTCGAAA8001.3973167E-69.3092431
CGCAAAC8101.6542963E-69.1943141
CGCCACT18000.09.1614781
CCGGGAT16350.09.1099631
CGGGAAT12301.6370905E-109.0821891
Produced by FastQC (version 0.11.2)