FastQCFastQC Report
Fri 10 Jun 2016
G95-M5_CAGATC_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M5_CAGATC_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4803819
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT2001.5433194E-518.6023271
CCGCGAT2501.0442805E-414.8818621
CGCCGAT1900.00432341214.6860481
CGCACGT2550.00204193512.7663041
CGGGAAT6703.45608E-1112.4941011
CGCAAAT3802.1070751E-512.2383731
CGCCAAT5052.1653068E-711.9717961
CGATTGG8800.011.62560754
GCGATTG9700.011.0269233
CCCGAAT8501.8189894E-1110.9425461
CGGTATT4256.609044E-510.9423182
GTCGATT4801.9323888E-510.6575831
CTTGAAT36250.010.6482291
CTCCGTT11350.010.2435731
GGTAATC10501.8189894E-1210.1867772
AGCGATT9651.8189894E-1110.1202262
AAATCGA7402.7643182E-810.054584
TACGATT3750.00315745019.9210352
TCCGATT6606.3381776E-79.8646662
CCGGAAT11850.09.8113541
Produced by FastQC (version 0.11.2)