FastQCFastQC Report
Fri 10 Jun 2016
G95-M5_CAGATC_L002_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M5_CAGATC_L002_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4803819
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAT2751.1083208E-414.7781651
CTCGAAT6809.094947E-1213.4470421
CGGGAAT8700.013.4298691
CTCGTAT3508.509793E-411.6114151
TCGGTTA4052.2732695E-411.2887442
CGATCAC6401.2317469E-711.1124871
CGCCAAT5502.7093101E-611.0836231
CTCGGAT13100.010.8580031
CTCGGTT8859.822543E-1010.3321911
AGATCGA6952.8849608E-69.7918474
GAGTCGA5352.0487286E-49.7848084
GCGATTG10257.4396667E-109.6846983
CGAGAAT12103.6379788E-129.6561861
CGATTGG10156.2264007E-99.2835184
AAATCGA7456.983437E-69.1346774
GATCGAT4600.00641410439.1041265
AAATCCG9803.4751793E-89.0809014
CTGGAAT56650.08.9673331
CGGGTTT8556.683549E-78.912271
TCGTATT5150.00206686188.8775562
Produced by FastQC (version 0.11.2)