FastQCFastQC Report
Fri 10 Jun 2016
G95-M5_R2_cat.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M5_R2_cat.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9627611
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAT7404.9312803E-911.2310511
CTCGAAT16200.010.26046851
CGCCAAT11650.010.2549631
CGCGCAT5804.5972385E-59.8513771
CTCGCAT11658.913048E-119.3632271
CGGCTAT7801.3693734E-69.3232131
CGCCTAT9753.630703E-89.0568361
CTCGGAT24400.08.9411141
CTTGGAT75950.08.8909421
CGGCGAT7801.3773137E-58.65726951
CGGAAAC17750.08.4865631
CGCCATT23300.08.4714911
CGGAAAT16101.8189894E-128.3884111
CTCGTAC12402.8448994E-98.3780031
CCGGAAC24250.08.3538191
CCGCAAT10601.4011312E-78.330581
CCGGAAT20800.08.2410551
CTCGGTT19600.08.2155721
CTCGAAC23550.08.1609931
CGACAAT10308.061961E-78.0689121
Produced by FastQC (version 0.11.2)