FastQCFastQC Report
Fri 10 Jun 2016
G95-M6_CTTGTA_L002_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M6_CTTGTA_L002_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7812714
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAT3957.2759576E-1219.20021
CGCGCAT3808.919653E-613.3054031
CGCCAAT9755.4387783E-109.852821
CGCGTTT4658.495058E-49.7859091
GCCGATC9202.190609E-89.342642
CCGCTAT9755.673428E-99.3342511
GTCGATT7156.445687E-69.1928231
CGCGGGT5604.5916936E-49.0286661
AATCGTG9301.6299418E-78.9616375
CGGGTAT7902.3477478E-68.9600931
CGCAAAT6301.522451E-48.8280291
CTCGAAC20300.08.7173321
CTTGGAT59750.08.6312531
CCGGAAT15850.08.6128341
CCGGTAT8851.1757511E-68.569581
CCGGCTT20300.08.4682661
CTCGTAT6652.7338683E-48.3633961
CTCGACT12851.0513759E-98.262811
CCGGAAC18500.08.1990041
CGCATAT5600.0046020068.1257991
Produced by FastQC (version 0.11.2)