FastQCFastQC Report
Fri 10 Jun 2016
G95-M7_AGTCAA_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M7_AGTCAA_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10357354
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT1856.8828485E-620.4269281
CGCGATT3251.0759322E-817.4414541
CGCGACT4603.3815013E-914.376561
CTCGCAT13300.011.0101911
CGCGGTT7602.91584E-910.5662321
CGCCGAT5901.320961E-610.4082131
CTCGAAT16800.010.4034471
CGGGAAT17150.010.1911321
CGCGAAT4651.4041498E-410.1585541
CGCCTAT10751.8189894E-1210.1065791
CGACAAT10801.8189894E-1210.0597891
CCCGAAT19150.09.8667941
TAAATCG6152.7594742E-69.8264133
CGCGTTT4852.1470936E-49.7396441
CTCGGTT20650.09.607581
CCGGCAT18850.09.5226341
CCCGGTT19800.09.3043111
TACGCGG4000.0054023279.2964029
CGCCCTT20500.09.2170291
CGGAAAT16950.09.1966371
Produced by FastQC (version 0.11.2)