FastQCFastQC Report
Fri 10 Jun 2016
G95-M7_AGTCAA_L001_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M7_AGTCAA_L001_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10357354
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAT12450.011.0932551
CGCCAAT12001.8189894E-1210.1812621
CGCCGAT5803.5948047E-510.0744111
AATTCGA14150.010.048064
CGCACAT16950.09.4017041
ATCGAGC13950.09.101836
CGCAAAT9351.3108001E-79.089991
CCGGAAT24350.08.9441661
CGGCAAT12552.5102054E-108.8885431
CGCAGAT24950.08.7290761
GTCGGTT13401.0004442E-108.7211321
TCGCGTA2902.0384327E-48.59376968-69
CTCGACT15603.6379788E-128.512761
CGCATAC7556.820962E-58.4428541
CGCCATT23400.08.3992581
CGGGAAT15955.456968E-128.325961
CGGGGAT26900.08.293771
CGCATTT13501.0513759E-98.2630531
CCGGACT17305.456968E-127.9832971
CGCGTCT11453.3691322E-77.8867561
Produced by FastQC (version 0.11.2)