FastQCFastQC Report
Fri 10 Jun 2016
G95-M8_AGTTCC_L001_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M8_AGTTCC_L001_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7811192
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTT5055.489419E-712.6522021
CGCGGTT6859.913492E-1012.4367141
CCCGTAT6859.913492E-1012.4367141
CGGATAC5502.026627E-712.0331593
CGCATAT4957.866325E-510.7565021
GTCGGAT8001.1323209E-810.6489361
CGCCAGT21050.010.1177551
CGCAAAT5853.8493876E-510.0118211
CTCGAAT11453.8198777E-119.7654011
CTCGTTT15750.09.127661
CCGAACT10053.9732186E-89.0065641
CGATCGG4550.00737392538.9509324
CGGGAAT13801.8189894E-118.8741141
CCGGCAT15600.08.8741131
CGGCAAC13902.1827873E-118.8102711
CTCGTAT6207.562028E-48.58785251
CGCAACT8101.6222617E-58.5454441
CCGGAAT19950.08.5405011
CCCGGAT18900.08.4515371
CGGCAAT8905.834472E-68.3755671
Produced by FastQC (version 0.11.2)