FastQCFastQC Report
Fri 10 Jun 2016
G95-M8_AGTTCC_L002_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M8_AGTTCC_L002_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7689233
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT7550.015.4000491
CGCCCAT17650.011.8576011
CGCAATA3551.5176907E-411.7904912
CAATCGA5152.7936585E-711.7393864
CGCCACT20900.011.5714041
CCGGCAT14500.011.546851
AATCGGG8107.2759576E-1211.482975
CGACAAT7753.8198777E-1111.4019971
CGCGCAT4155.1839597E-511.2067821
CCGCAAT7502.564775E-1011.1619551
CCGGAAT18100.010.5349941
CGACGGT3600.002249487210.3351431
GCGGAAT8109.767973E-1010.3351431
CCGAATT9802.5465852E-119.9660311
CCGCGTT4752.0358255E-49.7911891
CGCTAAT4308.9175266E-49.7342621
CGGTAAT4802.2621149E-49.6891981
AATCGAC7402.9680268E-79.4268985
CGACGAT4953.081564E-49.3955851
CCGGGTT17450.09.3282821
Produced by FastQC (version 0.11.2)