FastQCFastQC Report
Fri 10 Jun 2016
G95-M9_ATGTCA_L001_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M9_ATGTCA_L001_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6577091
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTT5001.5574733E-712.2792111
CCGGAAT15700.012.0325441
CGGCAAT7107.4578566E-1111.9732281
CTCGGTT14200.011.3080491
GTCGATT7009.156793E-910.7949111
GCGGGTT5708.4979547E-710.7712371
CGCACGT5801.0629738E-610.5855261
CCGGTTT10500.010.3451221
CGCCCAT14000.010.1202291
CGCCTGT17800.010.0823251
CGCGAAT3800.00310122489.9426811
CCGGGAT16450.09.7613551
CGCCTAT6858.533898E-79.6523851
CGACAAC9307.9671736E-109.6486771
CCGGATT11851.8189894E-129.565111
CGCCAAT8954.2055035E-99.4983161
CTCGAAC17000.09.4455471
CTCGCGT4550.00129943089.341751
CCCGAAT11757.2759576E-129.2445781
TCGGTTG7151.5373298E-69.2441042
Produced by FastQC (version 0.11.2)