FastQCFastQC Report
Fri 10 Jun 2016
G95-M9_ATGTCA_L001_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M9_ATGTCA_L001_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6577091
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAT3655.6083954E-513.115641
CGGGAAT10300.012.9104851
CGCGGAT4602.6979942E-612.7196361
CGCCTAT6104.3942237E-711.335831
CTCGTAT4704.690169E-511.3172781
CCGCGAT5451.7823802E-510.7358391
CGCGCAT3550.0088564310.4884061
TCGAATT8805.0478775E-89.6709552
CCGGGAT17850.09.5356781
CGCCATT14600.09.4724061
CGGGTAT6801.9619532E-59.38668251
CGGCATT12502.3646862E-119.3616521
CTCGACT10508.472853E-99.1184921
CGAATTC8556.5929817E-78.9208843
CGCCTGT18000.08.8652011
CCGCAAT7203.848364E-58.86521
CGCCACT16550.08.6777191
CTCGTAA6157.046598E-48.6489761
TTCGCTA5550.00268454698.6254462
AAGTCCG9453.0100637E-78.609144
Produced by FastQC (version 0.11.2)