FastQCFastQC Report
Fri 10 Jun 2016
G95-M9_ATGTCA_L002_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M9_ATGTCA_L002_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6486443
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTT3052.7305548E-413.2891051
CCGGAAT15150.010.7014571
CGCCCAT13350.010.6263071
AATCGGC5904.540425E-610.6161825
CGGCAAT7256.31062E-810.4823541
CGGGAAT10751.8189894E-1210.3685911
CGCCGAT4000.00265029110.1329421
CTCGGTT12450.09.7666911
CGATCAC9453.1977834E-99.6504211
CCGGGTT14200.09.6334311
CGCAAAT5352.8466273E-49.4700391
CGATTGA5603.3384876E-49.3207554
CCGAAAT12203.8198777E-119.136261
CCGAAAC10008.454663E-99.1196481
CGGGTAT6702.9253079E-59.0742761
AATCGAG5855.167416E-48.9224325
CGCCCGT6853.795948E-58.8755691
CCGGAAC15800.08.6578931
CGCCATT14109.094947E-128.623781
CTGCGAT15900.08.6034411
Produced by FastQC (version 0.11.2)