FastQCFastQC Report
Fri 10 Jun 2016
G95-M9_R2_cat.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M9_R2_cat.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13063534
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAT7756.730261E-1112.05413151
CGGGAAT22200.011.2215631
CGCGGAT9051.0935764E-89.7491221
CGCCGTT8554.7288268E-89.7122311
CTCGTAT10309.1677066E-109.5737371
CCGCAAT13101.0913936E-119.1121671
CGCATAT8554.908725E-79.1052161
CTCGACT21800.09.0467431
CGGGTAT12654.9112714E-119.0260411
CGCCTAT12101.9281288E-109.0073921
CGCCATT28850.08.8148591
CCCGAAT25900.08.6165591
CCGGAAT29800.08.5338491
CCGGGAT34800.08.5008191
CCGCGAT10701.651897E-78.2457521
CGAAGAT28450.08.2090971
CCGGCAT26150.08.1372431
CGGCATT24550.08.03336051
CGCCAAT19050.07.90074731
CCGGGTT32200.07.8977861
Produced by FastQC (version 0.11.2)