FastQCFastQC Report
Thu 23 Jul 2015
G96-M1_CCGTCC_L012_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG96-M1_CCGTCC_L012_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8717311
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC21150.025.0619143
ACGATCA20600.025.0153242
CACGATC26950.021.0356041
CGCATAT3901.1202974E-714.4160081
CGCGTGT7550.014.2728031
CCGGGAT20550.011.6275111
CGCCATT18550.011.6182931
CCGAAAT13000.011.5328071
CGGGGAT20950.011.4055051
CGATTGG51850.011.3885194
GCGACGT3752.3581744E-411.2444871
CGCAATT13400.011.1885441
CGCCGTT4801.7807019E-510.7369221
AATCGTG10400.010.7297655
GTTCGAT14900.010.3766231
ATTGGAC52150.010.341566
CCGGAAT29000.010.3397581
CTCGTAT14350.010.20595244-45
CTCGAAT25450.010.1251931
ACCCGTC15050.010.04202732-33
Produced by FastQC (version 0.11.2)