FastQCFastQC Report
Thu 23 Jul 2015
G96-M2_GTCCGC_L012_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG96-M2_GTCCGC_L012_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10213958
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCA27700.027.1195622
CGATCAC27750.026.4598713
CACGATC29400.026.4332451
AAGACGT63200.012.3492663
CGCCATT21500.012.0486421
GTTCGAT17350.011.9444751
AAAGACG74200.011.2412972
TCACAAG68100.011.0212196
ATTGGAC73000.010.9856476
AGACGTG66750.010.9345484
CTCGAAT27150.010.495411
AATTGGA92800.010.4124865
GAAAGAC95700.010.3943151
CGGAAAT22450.010.384911
CGAATGT35150.010.3716463
GAATTGG95250.010.3609614
ACGTGGT68250.010.3190996
GACGTGG69000.010.2795625
GTCGGTT23900.09.9716381
CGCAATT16200.09.91411
Produced by FastQC (version 0.11.2)