FastQCFastQC Report
Thu 23 Jul 2015
G96-M3_GTGAAA_L012_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG96-M3_GTGAAA_L012_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10274105
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCA25100.026.139752
CGATCAC26900.024.5462633
CACGATC29950.022.5361041
CGCCATT24350.013.4744871
CTCGAAT31250.013.0491551
CGACGAT7750.012.700791
CCGGAAT31350.012.5589961
CGGAAAT24500.012.2441051
CGGTAAT4406.747141E-611.7179911
TACGTCG3400.001402292510.9386889
CCGGCAT23100.010.7541741
TCACAAG67800.010.5608316
CGATTAG16550.010.3956334
CGGGGAT22050.010.2034211
CGCAATT12950.010.13447951
TAAGGCG21450.09.9697619
ATTGGAC65400.09.8819836
CGATTGG59400.09.7852244
CCGGGAT20250.09.7215931
CGCGGAT10204.9112714E-119.6501111
Produced by FastQC (version 0.11.2)