FastQCFastQC Report
Thu 23 Jul 2015
G96-M3_GTGAAA_L012_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG96-M3_GTGAAA_L012_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10274105
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCA23900.025.2063662
CGATCAC24300.024.7979323
CACGATC29550.021.4327091
CTCGAAT29100.012.2091521
CGCGTGT9150.011.8175551
GAATCGC16000.011.8092273
CGCCATT22950.011.2180341
AATCGCA19950.010.7606174
CGCCGTT7851.4209945E-810.4949351
CGCCAAT13900.010.3722421
TCACAAG65600.010.2080626
ATTGGAC66350.010.1697176
GAATTGG90600.09.985644
CGCTTAT6802.6976377E-69.8438251
CCACTAG30050.09.6865723
CGAATGT33800.09.6694773
CCGATAT16600.09.6157471
AATTGGA87350.09.5962665
CGCGGAT10259.695214E-109.5446321
AAGACGT58150.09.4844863
Produced by FastQC (version 0.11.2)