FastQCFastQC Report
Thu 23 Jul 2015
G96-M6_GATCAG_L012_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG96-M6_GATCAG_L012_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4795609
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAG7250.015.4416794
CTCGAAT13650.014.2783651
CGCCGTT3858.80428E-613.321921
CTCCGAT16400.013.1350891
CCGATTA8650.012.9302663
CGGCAAT10400.012.3291811
CGACAAT5853.097557E-812.2743851
CCCGAAT11800.011.7357081
TCGAATG14250.011.5173492
CGAATGT16100.011.3678283
TCGAAAT6309.60481E-811.314097
TAAGGCG12850.011.1434099
CGATCAC6006.3647167E-711.0152163
CGCGTGT3750.001398744110.9417371
CGCGTCT6702.0307198E-710.7171861
TCCGATT11550.010.6572872
CGGGCTT16200.010.447841
CTAGCCT23650.010.4096871
CGCGTTT3450.00938519710.4065441
CGGAATT11350.010.3932032
Produced by FastQC (version 0.11.2)