FastQCFastQC Report
Wed 12 Jul 2017
G97-M11_GTCCGC_L000_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG97-M11_GTCCGC_L000_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12991697
Sequences flagged as poor quality0
Sequence length67
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG16550.025.06350332
GTCACGT21850.018.84523829
CTCGTAT23200.018.14151844
CGCACAT23500.018.0415837
GATCGGA23200.017.4875131
TCACGTC24250.017.23096830
GTCCGCA27000.016.15411434
ATCTCGT26900.016.10096742
AGTCACG28000.015.57706428
ACGTCTG27750.015.49834715
CGTCCGC28650.015.01085533
TCCGCAC28250.014.8996135
TATGCCG29250.014.38939148
CCGCACA29750.014.35380536
ATCGGAA31750.012.8725712
CACGTCC32950.012.86649531
CGTATGC33700.012.48930746
CCGGAAT26150.012.0151711
TCTCGTA37550.011.85838143
GCACACG37600.011.76145311
Produced by FastQC (version 0.11.2)