FastQCFastQC Report
Wed 12 Jul 2017
G97-M12_GTGAAA_L000_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG97-M12_GTGAAA_L000_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16532363
Sequences flagged as poor quality0
Sequence length67
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAACG26100.021.73667135
AACGATC27450.020.5585938
AAACGAT29000.019.66996637
GTCACGT29600.019.37278229
CTCGTAT29950.019.14459444
ACGATCT29950.019.04650539
GATCGGA30800.017.4303531
GATCTCG34950.016.49594741
ATCTCGT35800.015.93348442
TCACGTG36400.015.83667330
CACGTGA37100.015.62007831
ACGTGAA37100.015.53800932
ACGTCTG36550.015.52138315
AGTCACG38450.015.30994728
TATGCCG38200.015.24962248
CGCGTAT3309.968062E-1013.8650541
CGATACT25250.013.7700861
CTCGAAT37550.013.1598011
CGTATGC43450.013.12613846
CGTGAAA44100.012.86463833
Produced by FastQC (version 0.11.2)