FastQCFastQC Report
Wed 12 Jul 2017
G97-M5_CAGATC_L000_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG97-M5_CAGATC_L000_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14499430
Sequences flagged as poor quality0
Sequence length67
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29700.016.2254683
CGTATGC37050.013.6676191
ATGCCGT39650.012.4611874
CTCGAAT30050.012.0802361
ACGATCA23400.011.9913632
CGATCAC22750.011.9317793
CGATACT18450.011.73687761
GCCGTCT43600.011.54138856
CACGATC28550.011.4327471
TAAGCCG14600.011.071835546
CCGGAAT27650.010.9222741
CCGTCTT51150.010.4340527
CTTAGAT35450.010.2400881
TGCCGTC49250.010.218025
CGACGAT6951.8189894E-1210.0952251
CTCGTCT38450.010.0758191
CTCGATT8800.010.052831
CCGGGAT22100.09.9383251
CGCGGAT10200.09.8693081
CTCGTTT26700.09.8256241
Produced by FastQC (version 0.11.2)