FastQCFastQC Report
Wed 12 Jul 2017
G97-M6_CTTGTA_L000_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG97-M6_CTTGTA_L000_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15686657
Sequences flagged as poor quality0
Sequence length67
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG27750.015.93939939
CTCGTAT29200.015.04289142
GATCGGA28700.014.6673031
ATCTCGT33300.013.55743440
GTAATCT34400.013.47848137
CGCCGAT7200.013.13355351
TATGCCG36500.013.1189463
ACGTCTG34650.013.11512415
CGATACT20600.012.43683161
CTCGAAT33750.012.3822581
CGTATGC42700.011.6442831
CCACTAG39700.011.52372553
CGATCAC22450.011.4118253
GCACACG43200.010.87173111
ACGATCA21800.010.7727452
ATGCCGT47250.010.7147784
ATTAACG15950.010.51678720
TAATCTC43600.010.35474538
ACTAGCC43900.010.21254355
CGCGTGT9950.010.1168171
Produced by FastQC (version 0.11.2)