FastQCFastQC Report
Wed 12 Jul 2017
G97-M7_AGTCAA_L000_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG97-M7_AGTCAA_L000_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18122773
Sequences flagged as poor quality0
Sequence length67
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG34500.016.8851153
ACGATCA31450.016.7771742
CACGATC36450.015.9004911
CGATCAC36000.014.7413243
CGTATGC42550.014.266191
CTCGAAT38150.012.4733911
CGATACT26600.012.38334261
GCCGTCT53900.011.8819576
ATGCCGT51300.011.8309464
TAAGCCG19000.011.076241546
GCATACG32100.010.92593556
CCGTCTT60150.010.7487427
ATTAACG30450.010.71764620
CGATTAG24200.010.5863864
TACGATA35000.010.45698659
CCGATAT18750.010.24928951
CGCGCAT8350.010.2288321
CGCAATT16750.010.1982981
CGCCATT32750.010.1524231
CCGGGAT27650.010.1495591
Produced by FastQC (version 0.11.2)