FastQCFastQC Report
Wed 12 Jul 2017
G97-M9_ATGTCA_L000_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG97-M9_ATGTCA_L000_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14541520
Sequences flagged as poor quality0
Sequence length67
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATC38900.021.9564531
ACGATCA36500.021.8089982
CTCGTAT26000.021.34868644
AATCTCG27100.020.48551641
GATCGGA25600.020.018111
CGATCAC40500.019.579583
ATCTCGT29700.019.102242
TATGCCG31300.017.63744748
ACGTCTG32350.017.06562415
TCTCGTA36900.015.7862943
TCGTATG36450.015.48032845
ACACGTC37300.014.79971313
CGTATGC37700.014.72414446
CGTCTGA38750.014.24630916
ATCGGAA37450.014.0890692
GCACACG40100.013.84223211
CGCCATT23800.013.7138871
TAAGCCG14600.013.36986846
CGATACT20450.012.97542161
ATTAACG47200.012.66521520
Produced by FastQC (version 0.11.2)