FastQCFastQC Report
Wed 1 Jun 2016
131127Wax_D13-6253_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename131127Wax_D13-6253_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences23215359
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTA9700.034.81702835
ACCGCGT11800.028.2514833
GCGTTAT13300.025.22904636
CGTTATA13800.023.99917237
TCTCGTA14100.023.33402344
GATCGGA18900.022.8890881
CCGCGTT15600.021.6490534
ATGCCGA20350.021.41397925
CGGTTCA19150.021.06003212
TATCTCG16950.019.79624442
ATCGGAA22650.019.4356942
AGCGGTT20950.019.2505810
AGACCGC17650.018.39387931
GAGCGGT22200.018.2710579
GACCGCG18000.018.03621932
GCGGTTC22900.017.71255311
CGAGACC19450.017.36376229
AATGCCG25350.017.27626424
ATCTCGT21000.015.770884543
AAGAGCG29000.015.7051397
Produced by FastQC (version 0.11.2)