FastQCFastQC Report
Wed 1 Jun 2016
131127Wax_D13-6256_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename131127Wax_D13-6256_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences20405520
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAT22200.031.40490732
GATCGGA25650.030.6598261
TCTCGTA22200.030.61978544
ACCGATG25100.028.0368633
CCGATGA25450.027.7368934
ATGCCGA28150.027.630625
CGATGAT25300.027.4707635
CGGTTCA27800.027.44184712
AGCGGTT27500.027.40393410
ATCGGAA29300.027.1454522
GAGCGGT28150.026.9359059
ATCTCGT26150.026.49451343
AATCTCG27200.025.55184742
GCGGTTC29500.025.46743611
AGACCGA27600.025.33940931
CGAGACC28750.025.2352129
TGCCGAG32250.024.38810226
CCGAGAC29750.024.02079828
AATGCCG33600.023.47306624
GAGACCG30700.023.41941830
Produced by FastQC (version 0.11.2)