FastQCFastQC Report
Wed 1 Jun 2016
131127Wax_D13-6257_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename131127Wax_D13-6257_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences19030034
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAAGTTATCTCGTA357370.18779262296641194Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAGT49050.037.53349335
TCTCGTA49950.037.51148244
ACCGCAA50600.036.98655733
AGACCGC52100.036.0471431
ATGCCGA59600.035.97080225
GACCGCA52350.035.87499632
TATCTCG53150.035.2940242
GATCGGA61600.035.283571
CGGTTCA58350.034.8515112
CCGCAAG54400.034.4830434
CGAGACC55200.034.18063429
AGCGGTT60000.034.1249710
ATCTCGT55600.033.738843
AATGCCG63700.033.72397224
ATCGGAA64950.033.717332
TGCCGAG63250.033.6883426
GAGCGGT60850.033.5339669
CCGAGAC56650.033.4595928
GCGGTTC61000.033.45150811
GAGACCG56900.033.23630
Produced by FastQC (version 0.11.2)