FastQCFastQC Report
Wed 1 Jun 2016
131127Wax_D13-6258_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename131127Wax_D13-6258_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences23342271
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTCCAGATCTCGTA251210.10762020542045801Illumina Paired End PCR Primer 2 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGT34000.035.94597232
GATCGGA43000.035.0544431
ACCGGTC35100.034.94359633
TCTCGTA37600.033.95283544
CGGTTCA42550.033.69268412
ATGCCGA43900.033.348125
CGGTCCA37100.033.29472735
GATCTCG39450.032.8576342
GCGGTTC44100.032.61367811
ATCGGAA47100.032.2448962
GAGCGGT44750.032.19189
CGAGACC40500.031.52164829
AGCGGTT46350.031.03048910
AGACCGG41350.030.97905231
TGCCGAG48350.030.32388926
CCGAGAC42600.030.17232128
CCGGTCC40950.029.95165334
GAGACCG43200.029.60197330
ATCTCGT43550.029.56416543
AATGCCG50200.029.46675724
Produced by FastQC (version 0.11.2)