FastQCFastQC Report
Wed 1 Jun 2016
131127Wax_D13-6259_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename131127Wax_D13-6259_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences18811789
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA25100.034.5483631
TCTCGTA23100.034.32793444
CGGTTCA24750.033.93393712
GAGCGGT25150.033.394239
ATGCCGA26450.032.28632725
GCGGTTC26450.031.75292611
ATCGGAA28200.031.1373162
AGACCGT26000.030.91799231
CGAGACC26900.030.53144329
GACCGTG26700.030.27059232
TCGGAAG29250.030.0894173
CGTGGAC26900.029.8835635
GAGACCG28100.029.2276130
CCGAGAC28150.029.17569728
TGCCGAG29450.028.7754826
AGCGGTT29800.028.41695410
GCCGAGA30850.027.68147327
CGGAAGA32250.027.3579544
ACCGTGG29600.027.30489233
AGAGCGG32250.027.1586958
Produced by FastQC (version 0.11.2)