FastQCFastQC Report
Wed 1 Jun 2016
D13_6255_Corrected.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameD13_6255_Corrected.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences16777583
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTACCGCATCTCGTA1018500.6070600276571423Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCG117850.042.54709235
GTACCGC119550.041.7955536
ACCGTAC120750.041.77912533
GACCGTA121450.041.6104432
CCGTACC120850.041.59960634
TCTCGTA119700.041.5784244
TACCGCA120200.041.4784537
ACCGCAT122400.041.1443738
ATGCCGA137750.040.91472625
CGAGACC125450.040.77226629
CCGCATC123450.040.68787839
AGACCGT124550.040.6449731
CCGAGAC127350.040.21554628
GAGACCG127900.040.1110930
GCCGAGA139950.040.03686527
CGCATCT125250.039.99825340
ATCTCGT124800.039.8617643
TGCCGAG141300.039.80931526
AATGCCG143200.039.4493124
CATCTCG127200.039.419542
Produced by FastQC (version 0.11.2)