FastQCFastQC Report
Sun 26 Mar 2017
G98_M1M2M5M6.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG98_M1M2M5M6.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences83464890
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA99600.031.71927844
GATCGGA121250.030.5581931
ATGCCGA120600.030.4235225
CGGTTCA124150.029.05653612
ATCGGAA133900.028.121972
GAGCGGT128550.028.1160969
AGCGGTT132400.027.15843610
ATCTCGT118200.026.83851843
AGACCGC95500.026.19106531
GCGGTTC140150.025.78896911
CGAGACC126250.025.64496429
AATGCCG144500.025.5573924
TATCTCG84850.025.39563642
CGCAAGT72350.025.05529235
TGCCGAG146350.025.04078326
ACCGCAA73900.024.64770533
CCGAGAC131450.024.5973328
GAGACCG134800.023.95371830
AAGAGCG158800.023.8077587
TCGGAAG161500.023.5821743
Produced by FastQC (version 0.11.2)