FastQCFastQC Report
Sun 26 Mar 2017
G98_M5M6.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG98_M5M6.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences39435554
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA71200.035.74081844
GATCGGA88600.033.8042261
ATGCCGA87800.033.4252525
AGCGGTT87500.032.7804910
CGGTTCA88650.032.40756612
GAGCGGT88850.032.3607149
CGCAAGT57800.032.30391335
ATCGGAA94250.031.9288922
ATCTCGT80350.031.86057343
CGAGACC82450.031.7361329
ACCGCAA60000.031.37362333
TATCTCG59850.031.306642
GCGGTTC93500.030.70173511
AATGCCG96800.030.63263724
CCGAGAC85200.030.5072128
GACCGCA61850.030.4704332
AGACCGC62150.030.42847831
TGCCGAG97100.030.3809326
GAGACCG87850.029.71095830
AAGAGCG107200.028.335317
Produced by FastQC (version 0.11.2)