FastQCFastQC Report
Sun 26 Mar 2017
131127Wax_D13-6252_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename131127Wax_D13-6252_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences20813977
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTTA13950.025.77063234
CGCTTAA14150.025.25240535
GATCGGA17700.024.6874121
TCTCGTA15200.023.22131244
CGGTTCA18000.023.05427712
ATGCCGA19300.022.2394625
ACCGCTT16950.021.85216733
ATCGGAA21250.021.3302482
AGACCGC17350.020.84605631
GAGCGGT20200.020.4286489
GACCGCT18050.020.2790432
AGCGGTT20450.020.17891110
AATCTCG17850.020.14007242
GCGGTTC22550.018.71094911
AATGCCG23300.018.51503824
ATCTCGT20000.017.86607643
TCGGAAG26300.017.6457123
CGAGACC21750.017.02962129
TGCCGAG25350.016.75992226
CCGAGAC22250.016.64693328
Produced by FastQC (version 0.11.2)