FastQCFastQC Report
Sun 26 Mar 2017
131127Wax_D13-6254_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename131127Wax_D13-6254_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences34448208
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAGCGATCTCGTA795420.23090315757498908Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCT108650.038.23705340
TCTCGTA110750.038.0431244
ATGCCGA137200.037.85433225
AGCGATC111400.037.41047339
CGATCTC111500.037.16241
GATCTCG117450.036.5963342
GACCGGC127800.036.24063532
GATCGGA146650.036.1360361
CGGTTCA140500.036.0286112
AATGCCG145000.035.87812424
GAGCGGT141900.035.8529859
AGCGGTT142000.035.82773610
ATCTCGT116850.035.8147643
CGAGACC130400.035.8020629
GCGGTTC143000.035.47985511
AGACCGG131800.035.4052331
CCGAGAC133250.035.08535828
CAGCGAT120400.035.0301738
ATCGGAA152800.034.9504972
TGCCGAG148850.034.7452426
Produced by FastQC (version 0.11.2)