FastQCFastQC Report
Sun 26 Mar 2017
131127Wax_D13-6261_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename131127Wax_D13-6261_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences25281165
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA19950.025.00620544
GATCGGA21800.024.7492451
GACCGAA21900.023.27699732
ATGCCGA23250.023.04980925
TATCTCG22350.022.6133942
ATCGGAA25250.021.9176772
CGGTTCA24600.021.31520312
AGCGGTT25200.021.17597610
CGAGACC25150.020.70213329
GAGCGGT25800.020.4137279
AGACCGA24900.020.3850531
CGAATGG26150.020.0770835
ATCTCGT27000.019.52570743
ACCGAAT26150.019.49392933
GCGGTTC27650.019.131811
CCGAATG27350.018.87757534
CCGAGAC27850.018.8515528
AATGCCG30150.017.84698524
GAGACCG29550.017.84074830
TGCCGAG33250.016.3796126
Produced by FastQC (version 0.11.2)