FastQCFastQC Report
Sun 26 Mar 2017
G98_M1M2M3M7.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG98_M1M2M3M7.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences103758709
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA158800.034.43580244
ATGCCGA200550.033.23103325
GATCGGA204750.032.787811
CGGTTCA204200.031.43717812
GCGATCT139400.030.94492540
AGCGGTT211050.030.53770610
GAGCGGT211700.030.4439479
ATCGGAA223600.030.4338052
AGCGATC142850.030.30432939
ATCTCGT181700.030.03584143
CGATCTC144150.030.00080341
CGAGACC203200.029.2809429
AATGCCG228750.029.22959524
GCGGTTC220800.029.19973811
GATCTCG152300.028.93896542
TGCCGAG232350.028.7110826
GACCGGC166150.028.25752832
CCGAGAC211950.028.14408128
GAGACCG215850.027.5144530
AAGAGCG256000.026.7684467
Produced by FastQC (version 0.11.2)