FastQCFastQC Report
Sun 26 Mar 2017
G98_M1M2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG98_M1M2.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences44029336
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTTA13600.024.3517635
GATCGGA34800.023.4800421
TCTCGTA29850.022.55484644
CGGTTCA36600.021.66016212
ATGCCGA38000.021.50172825
ATCGGAA42050.020.314932
AGACCGC34900.019.91548731
GAGCGGT40600.019.469069
ACCGCGT16950.019.41033233
AGCGGTT42200.018.67596610
GCGTTAT18650.017.64102636
ATCTCGT39200.017.39738543
CGCTTAA21000.017.22322735
GCGGTTC47350.017.03632511
CCGCTTA21200.016.95796834
AATGCCG49050.016.70223424
CGAGACC44300.016.13228229
TGCCGAG51150.016.05910926
CGTTATA21250.015.89272937
TCGGAAG54950.015.8605593
Produced by FastQC (version 0.11.2)