FastQCFastQC Report
Tue 7 May 2019
SRR8168075.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8168075.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34154256
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN744590.2180079694899517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG97450.015.1300843
GCATATC95500.011.8017056
TTCGATA34400.011.7399112
ATATCAC102900.011.6387168
CCACTAG132550.011.584083
CTCCGAT118950.011.3071991
TCGGTTA38750.011.2703142
CCCGGTT58100.011.089141
CTCGGTT78950.010.9006511
ATGCCGT147900.010.85864
CGTATGC137750.010.7540581
CTCGTTT111700.010.6517461
TCGGTTT89450.010.5521382
TCTAACG23700.010.5015232
CTCGTGT140050.010.4767091
TCGATAA35250.010.3903223
CTTAGAT151750.010.2577651
CACTAGC144700.010.125164
TCGCATT57950.09.9672752
GCTTTAA264100.09.9576275
Produced by FastQC (version 0.11.2)