FastQCFastQC Report
Tue 7 May 2019
SRR8168076.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8168076.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33603121
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1583750.47131038810353354No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN532770.15854777298811024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG115800.015.1652043
CCGATTA66950.013.4659153
TCCGATT83050.012.8918592
CTCGTTT110400.012.1832991
CTCCGAT114800.012.1669721
CGATTAG96650.011.9033424
ATATCAC92350.011.7980738
ATGCCGT165350.011.6719224
CGTATGC152750.011.6687941
GCATATC90550.011.616646
CTCGTGT129150.011.0699871
TGCCGTC183100.010.6435365
TCGTTTT131050.010.5707392
CCCGAAT119550.010.4247161
CTCGAAT123950.010.3581941
GCCGTCT190500.010.3039156
CTCGGTT85500.010.2309221
ATGCATA126550.010.1670074
CCACTAG120850.010.140963
TCGGTTT87850.010.1027682
Produced by FastQC (version 0.11.2)