FastQCFastQC Report
Tue 7 May 2019
SRR8168080.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8168080.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34805250
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACG37700.032.846813
TACGGCG41850.029.142996
ATACGGC67300.018.2620435
GATACGG75350.016.6840524
ACGGCGA102000.013.8428957
TCTAACG35600.013.7085092
CCGATTA69350.013.5950323
ACGTATA18150.013.58826418-19
ACACGAC42750.013.45151838-39
GGCGACC106050.013.4477949
CTCGAAT116300.013.2101351
TCCGATT85900.013.1106722
TACACGA40450.012.82375138-39
CTCCGAT121150.012.7200751
CGATCTA32800.012.668673552-53
CGATCAC79300.012.4807283
CGGCGAC120750.012.4707778
TCGCATT81000.012.4554762
AACGCGT86600.012.0786283
CCACGAT102750.011.7514251
Produced by FastQC (version 0.11.2)