FastQCFastQC Report
Tue 7 May 2019
SRR8168082.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8168082.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38876037
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN543070.139692736685069No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN401850.10336701757949249No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG95300.022.0346933
CGTATGC133650.015.7011851
ATGCCGT153500.014.8435964
GCCGTCT156750.014.7458596
CGATCAC97050.013.6504173
TGCCGTC183350.012.58103755
CTCGAAT124800.011.8003891
CCGTCTT195850.011.77815157
CTCCGAT134650.011.531191
CCGATTA79600.011.3903373
CGCGTAT12500.011.2921811
GCATATC110650.011.2514116
TCGGTTT100800.011.2327732
ATATCAC114100.011.1998598
CCACGAT124100.011.1465921
TCCGATT95650.010.9043172
CTCGGTT98500.010.7954011
CCACTAG136900.010.4661933
ACGATCA127200.010.3049892
ATTGGAC160200.010.2640336
Produced by FastQC (version 0.11.2)