FastQCFastQC Report
Tue 7 May 2019
SRR8168084.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8168084.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35414460
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC102000.019.220083
ACGATCA111250.017.5814112
CACGATC137550.015.8680731
TTACGAC28100.014.5567215
CCACGAT114550.014.20848751
CTCGAAT101950.013.2422411
TCTAACG27150.012.9882872
CCGATTA61650.011.896273
ATTGGAC185450.011.762666
CTCGCAT81250.011.752751
CTCCGAT118200.011.74008751
TGTTACG34150.011.5639953
AACGCGT58450.011.5019333
CGCGTAT9050.011.4351241
CTTAGAT166050.011.2465281
TCCGATT77950.011.0984462
TCGGTTT89900.011.0352772
TGCGCGA63250.010.704259
CTCGGTT91500.010.69323351
CTAACGC64750.010.6793381
Produced by FastQC (version 0.11.2)