FastQCFastQC Report
Tue 7 May 2019
SRR8168087.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8168087.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36113053
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC130300.024.0168533
ACGATCA133650.023.064822
CACGATC156750.020.9249741
TATGCCG73150.020.1055663
CCACGAT136150.018.3188381
ATGCCGT116350.013.7716244
CGTATGC109800.013.6290141
TCACAAG254650.012.9726
ATTGGAC240500.012.6606276
AACGCGT60800.012.5198113
GCCGTCT133000.012.3301726
CTAACGC67700.011.677781
CTCGAAT106950.011.3081621
TGCCGTC148250.010.9986595
GAATTGG334150.010.9404524
CTGGAAT540250.010.9055921
CTCCGAT124650.010.6839991
TAACGCG69850.010.629332
CTCGCAT83100.010.5896181
CGATTGG230400.010.5032054
Produced by FastQC (version 0.11.2)