FastQCFastQC Report
Tue 7 May 2019
SRR8168088.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR8168088.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39539927
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGCG56700.027.0871947
TGTTACG50100.020.9981523
TATGCCG88250.020.9677723
GTGCGTC77150.019.4823069
TTACGAC56700.019.2176075
AGGCGTA84500.018.6765798
CGATCAC89350.015.5059433
ATGCCGT132650.015.3307344
CGTATGC123450.015.2803691
ACGACTT82000.015.121317
GTTACGA75300.015.0939424
CTCGGCT196150.014.8211091
ACAGGCG118100.014.5552826
CTCGCAT145650.014.4693181
GCCGTCT146400.014.2439796
TAGCATA109650.014.1357898
TACGACT80750.013.9591246
TGCGTCG54850.013.83052510-11
GCGTCGA56550.013.70547610-11
TCGCATT135800.013.6615682
Produced by FastQC (version 0.11.2)